miR-34a Regulates Multidrug Resistance via Positively Modulating OAZ2 Signaling in Colon Cancer Cells

Figure 2

Manipulation of miR-34a expression affects chemosensitivity in CCa cells. (a) HCT-8/OR cells were transfected with miR-34a mimic or Mimic negative control (NC) as described in Materials and Methods. 48 h later, cells were collected, and the relative expression levels of miR-34a were assayed using RT-qPCR. HCT-8/OR cells with different transfections were incubated with different doses of oxaliplatin for 24 h, followed by cell viability assay (b) and apoptosis evaluation (c). Different superscript letters denote groups that are statistically different (). (d) HCT-8/OR cell-derived tumor xenograft model was established as described in Materials and Methods. Tumor volumes were measured every 4 days. and when comparing miR-34a mimic to Mimic NC. (e) HCT-8 cells were transfected with miR-34a inhibitor or Inhibitor NC as described in Materials and Methods. 48 h later, cells were collected and the relative expression levels of miR-34a were assayed using RT-qPCR. HCT-8 cells with different transfections were incubated with different doses of oxaliplatin for 24 h, followed by cell viability assay (f) and apoptosis evaluation (g). Different superscript letters denote groups that are statistically different (). (h) HCT-8 cell-derived tumor xenograft model was established as described in Materials and Methods. Tumor volumes were measured every 4 days. and when comparing miR-34a inhibitor to Inhibitor NC.

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