miR-34a Regulates Multidrug Resistance via Positively Modulating OAZ2 Signaling in Colon Cancer Cells

Figure 3

miR-34a inhibits the expression levels of signaling pathways associated with multidrug resistance. (a) Immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2 in HCT-8 and HCT-8/OR cells. β-Actin served as loading control. (b) 48 h after transfection with miR-34a mimic or Mimic NC, HCT-8/OR cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2. (c) 48 h after transfection with miR-34a inhibitor or Inhibitor NC, HCT-8 cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2. (d) Immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2 in HCT-116 and HCT-116/OR cells. (e) 48 h after transfection with miR-34a mimic or Mimic NC, HCT-116/OR cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2. (f) 48 h after transfection with miR-34a inhibitor or Inhibitor NC, HCT-116 cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2. (g) Immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2 in SW-480 and SW-480/OR cells. (h) 48 h after transfection with miR-34a mimic or Mimic NC, SW-480/OR cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2. (i) 48 h after transfection with miR-34a inhibitor or Inhibitor NC, SW-480 cells were subjected to immunoblotting analysis of the expression levels of MRP2, P-gp, BCRP, and Bcl-2.

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