miR-34a Regulates Multidrug Resistance via Positively Modulating OAZ2 Signaling in Colon Cancer Cells

Figure 4

Direct regulation of OAZ2 expression by miR-34a. (a) The binding site of miR-34a in the OAZ2 3UTR was analyzed using miRanda database. (b) Relative expression levels of miR-34a and OAZ2 mRNA in clinical CCa samples were evaluated using RT-qPCR, followed by Pearson chi-square test. (c) Immunoblotting analysis of OAZ2 expression in HCT-8 and HCT-8/OR cells with different transfections. (d) RT-qPCR analysis of OAZ2 mRNA expression in HCT-8 and HCT-8/OR cells with different transfections. (e) Simplified structure of the potential binding site of miR-34a onto the wild-type (WT) or mutant (Mu) OAZ2 3UTR. (f) 0.5 μg of pGL3-OAZ2 WT or pGL3-OAZ2 Mu, along with 0.05 μg of pRL-SV40 plasmid and miR-34a mimic, was cotransfected into HeLa cells. 48 h later, cells were harvested and subjected to luciferase activity measurements.

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