TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

Figure 1

Characteristics of NK cells in S. japonicum-infected TLR3^−/− mice. Female C57BL/6 wild-type mice (WI) and TLR3^−/− mice (TLR3 KO) were infected with S. japonicum cercariae or not and sacrificed after 5 weeks. Splenic lymphocytes were isolated and stained by different fluorescent-labeled antibody and analyzed by FACS. (A/B) CD19⁻ cell population was gated firstly. The percentage of CD3⁻NK1.1⁺ NK cells was analyzed. (a) A representative result is shown. (b) Statistical results of the percentage of NK cells in the spleen were calculated from FACS data. One syndrome represents an independent experiment. (c, d) The expression of MHC II, CD69, NKG2A/C/E, and NKG2D on NK cells of mice from both the normal and infected WT and TLR3 KO mouse spleen were detected. (c) A representative result is shown. (d) Statistical results of at least three independent experiments are shown. (e, f) Splenic lymphocytes were stimulated with PMA plus ionomycin for 5 hours, stained by the means of intracellular staining, and analyzed by flow cytometry. The expressions of IFN-γ, IL-4, IL-5, and IL-17 on NK cells from different groups are shown. (e) A representative result is shown. (f) The statistical results of at least three independent experiments are shown. Data are shown as mean + SEM of 3–5 samples from three independent experiments. and ; ns for . N: normal; INF: infected.

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