TLR3 Modulates the Response of NK Cells against Schistosoma japonicum

Figure 4

Expression of TLR3 on different spleen cell subsets. (a) Single spleen cell suspensions were separated, the population of small size live cell was gated firstly, and the expression of TLR3 on CD4⁺ T cells, CD8⁺ T cells, NKT cells, and γδT cells in CD3⁺ cell population, CD3⁻CD19⁺ B cells, and CD3⁻CD19⁻NK1.1⁺ NK cells was analyzed by flow cytometry. Statistical analysis of expression of TLR3 on different lymphocyte subsets of the spleen is shown in (b). (c) Splenic lymphocytes were gated on the total live cell population firstly; the expression of TLR3 on different myeloid subsets containing CD11b⁺Ly6G⁺ neutrophils, CD11b⁺F4/80⁺ macrophages, CD11c⁺B220⁺CD11b⁻ plasmacytoid dendritic cells (pDCs), and CD11c⁺B220⁻CD11b⁺ conventional dendritic cells (cDCs) is shown in (d). Data represent means ± SD of three independent experiments with 3–5 mice per group. ; . N: normal; INF: infected.

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