Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

Figure 2

CTX and CB are able to modulate the production of cytokines by DCs incubated with LPS in vitro. DCs derived from BALB/c mice bone marrow were stimulated with CTX, CA, or CB (250 ng/mL); LPS (250 ng/mL); and LPS + CTX, CB, and CA (250 ng/mL + 250 ng/mL). After 18 hours, the cell supernatants were collected for detection of IL-12 (a), TNF-α (b), IL-6 (c), and IL-10 (d) by ELISA. All samples were performed in quadruplicate and the results were expressed as the mean of the cytokine concentration ± SD. The dashed line represents the detection limit of each cytokine determined from the standard curve. Statistical analyses were performed by one-way ANOVA, followed by Tukey’s test (GraphPad Prism 5.0, GraphPad Software). —groups of cells incubated with LPS compared with DCs maintained in culture medium or DCs incubated with CTX, CB, or CA. —LPS + CTX and LPS + CB groups compared with the LPS group.

(a)

(b)

(c)

(d)