Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

Figure 4

Inhibitory effect of CTX and CB on the activation of NF-κB and MAPK signaling pathways on DCs incubated with LPS in vitro. DCs derived from mice bone marrow were stimulated with CTX; CB (250 ng/mL); LPS (250 ng/mL); LPS + CTX; and LPS + CB (250 ng/mL and 250 ng/mL) for 15 min. After this, total cell extracts were analyzed by Western blotting with anti-phospho-NF-κBp65 and total NF-κBp65 (a); phospho-p38 and total p38 (b); phospho-ERK1/2 (p44/42) and total ERK1/2 (p44/42) (c); and β-actin antibodies. Representative blots were shown and the densitometric analysis of bands was performed by ImageJ software, version 1.44. Values of phospho-ERK1/2, phospho-p38, and NF-κBp65 were normalized to total ERK1/2, p38 and NF-κBp65, and β-actin and were expressed in arbitrary units ± SD of three independent experiments. —groups of cells incubated with LPS compared with DCs maintained in culture medium or DCs incubated with CTX or CB. —LPS + CTX and LPS + CB groups compared with the LPS group.

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