Research Article
Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors
Figure 7
Role of FPRs in the modulatory effect of CTX and CB on cytokines and eicosanoids secretion by DCs incubated with LPS. DCs differentiated in vitro were incubated or not with Boc-2 (100 μM) for 15 minutes. After this, the cells were centrifuged and added with the culture medium containing LPS (250 ng/mL), LPS + CTX, or LPS + CB (250 ng + 250 ng/mL) for 18 h. After this, the cell supernatants were collected for measuring of IL-12 (a), TNF-α (b), IL-6 (c), IL-10 (d), PGE2 (e), and LXA4 (f) secretion by ELISA. The results were expressed as the mean of the cytokine concentration of each sample in quadruplicate ± SD. The dashed line represents the detection limit of the each assay. —groups of DCs incubated with LPS compared with cells maintained in medium culture; —LPS + CTX and LPS + CB groups compared with the LPS group. The data are representative of two independent experiments. Statistical analyses were performed by one-way ANOVA, followed by Tukey’s test (GraphPad Prism 5.0, GraphPad Software).
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