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Journal of Immunology Research
Volume 2018, Article ID 7948068, 9 pages
Research Article

Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Correspondence should be addressed to Xichun Wang; nc.ude.uaha@nuhcixgnaw and Jinjie Wu; nc.ude.uaha@jjw

Received 12 October 2017; Accepted 6 February 2018; Published 5 April 2018

Academic Editor: Jacek Tabarkiewicz

Copyright © 2018 Yu Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The current study aimed at investigating the effects of flavonoids from Astragalus complanatus (FAC) on the proliferation, the contents, and gene expression levels of cytokines, secretion of surface stimulating factors, cell cycle, and the expression level of the NF-κB signalling pathway in RAW264.7 cells. Our results revealed that compared with control group, the contents of IL-6, IL-1β, TNF-α, and NO and the mRNA expression levels of IL-6, IL-1β, TNF-α, and iNOS in FAC-treated groups significantly increased (). Moreover, FAC induced macrophage activation to release the above-mentioned mediators partly involved in NF-κB/MAPK signalling pathways. Therefore, FAC regulates immune function in RAW264.7 cells via activating the NF-κB signalling pathway. FAC could be applicable for agriculture, drug research, and food industry as a potent immune-modulatory agent.