Hepatitis B Vaccination Induced TNF-α- and IL-2-Producing T Cell Responses in HIV− Healthy Individuals Higher than in HIV+ Individuals Who Received the Same Vaccination Regimen

Figure 2

Gating strategies for flow cytometric analysis. The gating strategy used to determine cytokine-producing and CD107a-expressing memory T cells (a–c). Memory T cells were identified from lymphocyte population (a) and double positive population of CD3 versus CD45RO plot (b). Memory CD4+ or CD8+ T cells were then identified from CD4 versus CD8 plot (c). Cytokine production and expression of surface CD107a of CD45RO+CD4+ or CD8+ memory T cell subpopulation were then evaluated (d).