Hei-Gu-Teng Zhuifenghuoluo Granule Modulates IL-12 Signal Pathway to Inhibit the Inflammatory Response in Rheumatoid Arthritis

Figure 7

The effects of HGT on cytokine expression after IL-12Rβ1 knockdown. Firstly, the U937 cells were first treated with LPS and the other without LPS, and then all cells were transfected with IL-12Rβ1 siRNA. 48 h later, the U937 cells were then treated with HGT or without HGT for 48 h. (b) Western blot of the protein of IL-12Rβ1 and STAT4; (a, c, d, e, f) IL-12, IFN-γ, IL-4, IL-13, and IL-5 productions were determined by ELISA. The data are represented as the mean ± SD (); , when compared with the LPS group.

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