Sample Aging Profoundly Reduces Monocyte Responses in Human Whole Blood Cultures

Figure 1

Cytokine release in whole blood culture supernatants (as % of the response at , average plus SD). The whole blood of 3 donors was stimulated for 24 hours with LPS (a), PMA/ionomycin (b), or SEB (c). The whole blood of 5 donors was stimulated for 24 hours with LPS (d). The -axis indicates the sample age (time span between blood collection and start incubation). The blood was stored at room temperature (continuous line), 4°C (dashed line (a–c)), or 37°C (dashed line (d)).

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