Sample Aging Profoundly Reduces Monocyte Responses in Human Whole Blood Cultures

Figure 2

Cell viability (cell counts, annexin V, and MMP) and TLR4 expression in whole blood cultures (average plus SD). In whole blood cultures of 6 donors, absolute cell counts (T-cell, monocyte and granulocyte numbers (a)), apoptosis (annexin V-positive T-cells and monocytes (b)), mitochondrial function (MMP for T-cells and monocytes (c)), and TLR4 expression (TLR4-positive monocytes (d)) were quantified. The -axis indicates the sample age (time span between blood collection and start incubation). The blood was stored at room temperature (continuous lines) or 4°C (dashed lines).

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