Sample Aging Profoundly Reduces Monocyte Responses in Human Whole Blood Cultures

Figure 4

Cytokine release in whole blood culture supernatants; RPMI effect (as % of the response at , average plus SD). The whole blood of 3 donors was stimulated for 24 hours with LPS in the absence of RPMI (a) or diluted 1 : 1 with RPMI during aging (b). The -axis indicates the sample age (time span between blood collection and start incubation). The blood was stored at room temperature.

(a)

(b)