Research Article
Effect of CXCR2 Inhibition on Behavioral Outcomes and Pathology in Rat Model of Neuromyelitis Optica
Table 1
Immunohistochemistry markers.
| | Marker | Working dilution (clone) | Source company | Fixation/time | Detection | Chromogen |
| | Human IgG | 1 : 1000 | Vector Labs | PFA, 1 h | Elite ABC-HRP (Vector Labs) | DAB | | Neutrophils | 1 : 250 (His48) | BD Pharmingen | PFA, 15 min | Alk. phos. anti-ms IgM (Sigma) | BCIP+NBT | | Aquaporin-4 | 1 : 250 (H-80) | Santa Cruz Biotechnology | PFA, 1 h | Elite ABC-HRP | DAB | | GFAP | 1 : 250 | Dako USA | PFA, 1 h | Elite ABC-HRP | DAB | | Macrophage/microglia (rabbit anti-iba1) | 1 : 1500 (IHC) | Wako Lab. Chem. USA | PFA, 1 h | Elite ABC-HRP | DAB | | T cells (CD3) | 1 : 60 (sp7) | GeneTex, Inc. | PFA, 1 h | Elite ABC-HRP | DAB | | C4d | 1 : 1000 | American Research Products Inc. | PFA, 1 h | Immpress anti-rabbit alk. phos. (Vector Labs) | BCIP+NBT | | Anti-C5-C9 | 1 : 1000 (#204903) | Calbiochem | Acetone, 10 min | Immpress anti-rabbit alk. phos. | BCIP+NBT |
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