Novel Application of Radotinib for the Treatment of Solid Tumors via Natural Killer Cell Activation

Figure 3

Radotinib enhances cytolytic activity of NK cells against various cancer cells expressing Fas receptor. To further explore the involvement of the Fas receptor in cytolysis, various cancer cell lines were used to analyze the radotinib-enhanced cytolytic activity of NK cells. (a) Surface expression of Fas on various cancer cells, including A549, NCI-H460, A375, SK-MEL-5, MDA-MB-231, and MCF-7, was determined by staining with PE-conjugated Fas antibody (solid line). PE-conjugated mouse IgG antibody was used as an isotype control (dotted line). (b) The effect of radotinib on NK cytolytic activity against various cancer cell lines was determined by cytotoxicity assays. Radotinib-treated NK cells were used as effector cells, and cancer cell lines were used as target cells (E : T ). Data are reported as . All values were analyzed by unpaired Student’s -tests using GraphPad Prism 5. and . All data presented are representative of three independent experiments.

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