Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma

Figure 5

CTL activity. T cells cocultured with AAH-DCs, GFP-DCs, or blank DCs for 72 h were regarded as effector cells, respectively. HepG2 cells were used as target cells. On the other hand, AAH-DCs, GFP-DCs, and blank DCs were applied as vaccines to immunize C57BL/6 mice, respectively. The experiments were performed in triplicate, and the bars represent the mean ± SD. (a) The effect of T cells cocultured with DCs on the proliferation of HepG2 cells by CCK-8 analysis. , when compared with other groups. (b) The proapoptosis effect of T cells cocultured with DCs on HepG2 cells by FCM. , when compared with the control groups. (c) The lysis effect of CTLs derived from immunized mice against HepG2 cells. , when compared with other groups except the AAH group. According to our results, although there was no statistical differences between the AAH-DCs group and the AAH group, the lysis effect of the AAH-DC vaccine against HepG2 cells was stronger than that of the AAH vaccine. (d) The picture of apoptosis of target cells by FCM.

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