Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions

Table 1

Oligonucleotide sequences used for Q-PCR analysis.

Transcript	Sequence (5-3 direction)		Gene accession no.	Product size
FoxP3	F	AGGAGGATGGACGAACAGG	NM_014009.3	76 bp
	R	CACATCCAGGGCCTATCATC
CD28	F	CATGGCCCAAGTCTGTCTTT	NM_006139.3	63 bp
	R	TGTATGTCGGGCATGCTACT
CTLA-4	F	CCGTGCCCAGATTCTGAC	NM_005214.4	60 bp
	R	AAACAACCCCGAACTAACTGC
GITR	F	GACCGAAGACGCCAGAAG	NM_004195.2	95 bp
	R	CTCACACCCACAGGTCTCC
GAPDH	F	GCATCCTGGGCTACACTGA	NM_002046.5	79 bp
	R	CCAGCGTCAAAGGTGGAG