Comparison of Inhibitory Effects of Safflower Decoction and Safflower Injection on Protein and mRNA Expressions of iNOS and IL-1β in LPS-Activated RAW264.7 Cells

Figure 1

Flowchart on the process for producing safflower injection and the five samples obtained in the research [8]. Safflower: the 20 kg dried herb. Supernatant 1: the water decoction, and 20 ml of it was obtained as sample 1. 20 ml of each of the extracted supernatants 2, 3, and 4 was obtained as sample 2, sample 3, and sample 4, respectively. Supernatant 5: the 40000 ml safflower injection, and 20 ml was sampled as sample 5. The filtrate was concentrated to a relative density of 1.10–1.14 for supernatant 1, 1.16–1.20 for supernatant 2, and 1.02–1.04 for supernatant 3.