Comparison of Inhibitory Effects of Safflower Decoction and Safflower Injection on Protein and mRNA Expressions of iNOS and IL-1β in LPS-Activated RAW264.7 Cells

Figure 2

High-performance liquid chromatography profiles of the reference and five samples. Sample 1: the safflower decoction; sample 5: the safflower injection. Performance temperature was 25°C. Mobile phase A was gradient elution with acetonitrile, and mobile phase B was aqueous trifluoroacetic acid. The detection wavelength was 223 nm. 10 μl Hydroxysafflor yellow A (HSYA) was the control solution. Run time was 70 min. According to reference fingerprint, sample 5 showed 11 characteristic absorption peaks and peak 9 was confirmed as HSYA [8].