Absence of Nonclassical Monocytes in Hemolytic Patients: Free Hb and NO-Mediated Mechanism

Figure 1

The absence of nonclassical monocyte subset in SCD and PNH patients. (a) Using flow cytometry, total leukocytes were gated as CD45⁺ and monocytes were gated as CD14⁺CD11c⁺ cells. Monocyte subsets were identified as CD14^dimCD16⁺ nonclassical (red gated), CD14⁺CD16⁺ intermediate (blue gated), and CD14⁺CD16^- classical (green gated). (b) Representative flow cytometry plots of 3 individuals each from healthy, SCD, and PNH patients showed profiles of the nonclassical subset (red gate on both CD14 vs. CD11c plot and CD14 vs. CD16 plot). (c) Scattered dot plot showing frequencies of nonclassical monocyte subsets in healthy, SCD, and PNH patients (). Each dot represents percent-positive cells for an individual. The Mann–Whitney test was used for the comparison between the groups (). (d) Representative histogram plots showing the expression of CD11c on monocytes of SCD, PNH, and healthy individuals.

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