The Intracellular Growth of M. tuberculosis Is More Associated with High Glucose Levels Than with Impaired Responses of Monocytes from T2D Patients

Figure 2

Cytokine production after TLR-specific stimulation or M. tuberculosis infection and intracellular growth control in monocytes from T2D patients and healthy subjects. Cytokine production elicited by activation through TLRs or M. tuberculosis infection was measured in the culture supernatants. Interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), and tumor necrosis factor alpha (TNF-α) protein levels were measured by a CBA-based assay and reported as pg/ml. Monocytes from healthy subjects (open circle, ) and T2D patients with (closed circle, ) were stimulated with (a) Pam3Cys or (b) LPS or infected with (c) M. tuberculosis. Mann-Whitney test, healthy subjects vs. T2D patients with . Monocytes from T2D patients and healthy subjects were infected with M. tuberculosis (MOI of 10) for 1 h; after the indicated time, cells were lysed with 1% SDS, and the mycobacteria were serially diluted and quantified in 7H10 agar media after 21 incubation days, which was reported as CFU. (d) M. tuberculosis growth in healthy subjects (open circle, ) and T2D patients with (closed circle, ). The median is represented by a horizontal line.

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