Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis

Table 1

Primer sequences, restriction enzymes used, and restriction digestion patterns for genotyping of TLRs genes.

Gene	SNP	Primer sequences	Restriction enzymes	Restriction temperature (°C) (time)	Length of the restriction fragments
TLR2	rs5743708	F: 5-CATTCCCCAGCGCTTCTGCAAGCTCC-3	Msp I	37°C (overnight)	Allele G—
		R: 5-GGAACCTAGGACTTTATCGCAGCTC-3			Allele A—210 bp
TLR6	rs5743810	F: 5-GCATTTCCAAGTCGTTTCTATGT- 3	Ava II	37°C (3 hours)	Allele C—
		R: 5-GCAAAAACCCTTCACCTTGTT- 3			Allele T—210 bp
TLR9	rs5743836	F: 5-CTGCTTGCAGTTGACTGTGT -3	Mva I	37°C (3 hours)	Allele C—
		R: 5-ATGGGAGCAGAGACATAATGGA-3			Allele T—
	rs187084	F: 5-TATCGTCTTATTCCCCTGCTGGAATGT-3	Afl II	37°C (1 hour)	Allele T—
		R: 5-TGCCCAGAGCTGACTGCTGG-3			Allele C—145 bp

F: forward standard; R: reverse standard; TLR: toll-like receptor; °C: degrees Celsius; bp: base pair.