Research Article

Blockade Effects of Anti-Interferon- (IFN-) γ Autoantibodies on IFN-γ-Regulated Antimicrobial Immunity

Figure 4

Anti-IFN-γ autoAbs reduce the IFN-γ-induced bacterial killing capability of differentiated macrophages. (a) PMA-differentiated macrophages were treated with IFN-γ in the presence or absence of anti-IFN-γ autoAb-positive patient serum (PS) and incubated with SYTO 16 Green-stained heat-killed M. tuberculosis (HKMT). (b) Following the flow cytometry analysis, bacterial killing capability was analyzed by measuring decreases in the mean fluorescence intensity (MFI). All histograms show representative data. ns: not significant. .