pDC Activation by TLR7/8 Ligand CL097 Compared to TLR7 Ligand IMQ or TLR9 Ligand CpG
Ligands of TLR7 and TLR9 can significantly increase the release of IFN-α/TNF-α/IL12p70/IL-6 from pDCs. This effect is stronger for TLR7 ligands than for TLR9 ligands. Supernatants from the pDC culture medium were harvested after pDC activation with TLR7 ligands (IMQ, CL097, and CL075) and TLR9 ligands (CpG A, CpG B, and CpG C) for 24 and 48 hours. The concentration of each ligand was 1.5 μM. Cytokine release was assessed by ELISA and CBA. Data shown are expressed as (/group) and represent three independent experiments with similar results. Statistical significance of differences between groups was determined; ,, and .
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