Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF

Figure 5

Hsp72-IgG complexes induce CXCL8 secretion from in vitro MDM culture. MDM cell culture supernatant was analysed for CXCL8 (a) and CCL18 (b) by ELISA after treatment with 1 μg/ml IgG isolated from BALf of IPF  ng/ml Hsp72 (IPF patient , repeated twice) for 24 hours. 1 μg/ml of BALf IgG did not lead to any overt fold change in CXCL8 secretion in comparison to untreated cells, but preincubation of IgG with Hsp72 led to increased CXCL8 secretion ((a), ). A 4-fold increase in MDM CCL18 secretion was seen in response to BALf IgG, with Hsp72 preincubation having no effect ((b), ). Statistical tests to compare BALf IgG with preincubated BALf IgG used a Wilcoxon test; no statistical comparison was made with untreated cells.

(a)

(b)