Local Administration of Caloric Restriction Mimetics to Promote the Immune Control of Lung Metastases

Figure 1

Effects of CRM aerosolization on body weight, on the growth of experimental B16 lung metastases, and on lung histology. Body weights (a) and number of macroscopic lung metastatic foci (b) of mice (9-10 mice/group) treated starting 1 day after i.v. injection of B16 melanoma cells with aerosolized saline or combined CRMs for 3 weeks. Representative images showing histopathological evaluation of hematoxylin- and eosin-stained lung tissue sections (c) and IHC analysis of LC3B staining in tumor nodules (d) (magnification: ×200) of saline and CRM-treated mice. by Student’s -test.

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