Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses

Figure 2

Activated HMCs process antigen. (a) HMCs were treated with or without IFN-γ for 48 h, incubated with DQ-OVA for 48 h at 37°C in medium, washed, fixed, and stained with DAPI. The arrows indicate DQ-OVA processing analysed via confocal microscopy. (b) HMCs were pretreated with IFN-γ or medium alone for 48 h, incubated for 24 and 48 h with fluorochrome-labelled ovalbumin (DQ-OVA), and analysed via flow cytometry. The data in (b) were analysed by one-way analysis of variance (ANOVA) followed by Tukey’s HSD post hoc test. The data are representative of three independent experiments, and the error bars represent the . Con: HMCs treated without IFN-γ. vs. Con.

(a)

(b)