3<i>β</i>-Acetyloxy-oleanolic Acid Attenuates Pristane-Induced Lupus Nephritis by Regulating Th17 Differentiation

<div>AOA dose-dependent inhibition of Th17 differentiation. Native CD4<sup>+</sup>CD25<sup>-</sup> T cells were subjected to Th17 cell differentiation protocols as described in Materials and Methods. (a) Flow cytometry analyzing intracellular IL-17A in native CD4<sup>+</sup>CD25<sup>-</sup> T cells with AOA (0.625, 2.5, and 10 <i>μ</i>M) under mouse Th17 cell differentiation conditions. (b) The rate of inhibition was calculated vs. DMSO group. (c–e) <i>IL-17A</i>, <i>IL-17F</i>, and <i>IL-22</i> expression was quantified and normalized to GAPDH. The <i>in vitro</i> differentiation and quantitative real-time PCR were repeated 3 times with consistent results. 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Journal of Immunology Research

fig2

Figure 2

Figure 2: 3<i>β</i>-Acetyloxy-oleanolic Acid Attenuates Pristane-Induced Lupus Nephritis by Regulating Th17 Differentiation 

Journal of Immunology Research

3β-Acetyloxy-oleanolic Acid Attenuates Pristane-Induced Lupus Nephritis by Regulating Th17 Differentiation

Figure 2