TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of Schistosoma japonicum-Infected C57BL/6 Mice

Figure 1

Schistosoma japonicum infection promotes CD3⁺ T cell accumulation in mesenteric lymph nodes. Female C57BL/6 mice were infected with S. japonicum cercariae per mouse. The mice were sacrificed 6 weeks after infection. The tissues and single cell suspensions were harvested. (a) Representative images of mesenteric lymph nodes. (b) Single mononuclear cell solutions were stained by trypan blue, and the absolute numbers were counted under a microscope (15/20). (c) Flow cytometric analysis of CD3 expression in MLN cells of normal and infected mice is shown. (d) Average percentages of CD3⁺ T cells were calculated from the FACS analysis (15/20). (e) The absolute number of CD3⁺ T cells evaluated by flow cytometry after staining with specific antibodies (15/20). (f) Flow cytometric analysis of CD25 and CD69 expression in MLN cells is shown. The numbers represent the expression of cells in each subset. (g) Average percentages of CD25 and CD69 expressions in the CD3⁺ T cells were calculated from FACS data. Data was from three independent experiments with 5 mice per group and shown as the . , .

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