TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of Schistosoma japonicum-Infected C57BL/6 Mice

Figure 2

Expression of TLRs in the MLN of S. japonicum-infected mouse. Six weeks after S. japonicum infection, the mice were sacrificed. Single mononuclear cell suspensions from normal and infected mice were prepared as described in Materials and Methods. Total RNA was collected and purified, and cDNA was synthesized. (a, b) The relative mRNA expression of TLRs and β-actin genes was detected. Data was from three independent experiments with 5 mice per group and shown as the . , ^ns. (c, d) Single cell suspensions of MLN cells were prepared, and the expression of TLR2, TLR3, TLR4, and TLR7 on CD4⁺ or CD8⁺ T cells was detected by flow cytometry after staining with specific antibodies. (c) The numbers represent the expression of cells in each subset. (d) Average percentages of TLR2, TLR3, TLR4, and TLR7 on CD4⁺ or CD8⁺ T cells were calculated from FACS data. Three independent experiments (5–6 mice per group) were performed, and one representative result is shown. , ^ns.

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