The Nod2 Agonist Muramyl Dipeptide Cooperates with the TLR4 Agonist Lipopolysaccharide to Enhance IgG2b Production in Mouse B Cells

Figure 3

Combined effects of LPS and MDP on the expression of germline transcripts and AID mRNA. Resting B cells were stimulated with MDP (10 μg/mL) and iE-DAP (10 μg/mL) in the presence or absence of LPS (1 μg/mL). After 2.5 days of culture, RNAs were isolated, and the levels of germline transcripts and AID mRNA were measured by RT-PCR. The levels of germline transcripts and AID mRNA were measured by semiquantitative RT-PCR with 1/5 and 1/25 diluted cDNA (lower panel). The graphs show relative transcript levels normalized to the expression of β-actin cDNA by ImageJ (NIH, Bethesda, MD, USA) analysis. Densitometric data are averages of two independent experiments with ranges (bars).