Journal of Immunology Research / 2019 / Article / Fig 3

Research Article

Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®)

Figure 3

Evaluation of anti-TRCP-KLH antibodies and titration of antigen from mouse and rabbit sera. Before and after immunization protocols (see Figure 1), blood samples were taken to detect specific IgG anti-TCRP-KLH antibodies in the serum of animals immunized with the bioconjugate. An indirect ELISA was performed sensitizing plates from to μ g/mL of TCRP-KLH. (a) A pool of mice sera was diluted 1 : 1,000. (b) Serum from rabbit was diluted 1 : 10,000. Technical triplicates were carried out. .
(a) Mouse
(b) Rabbit

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