Research Article
Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®)
Figure 3
Evaluation of anti-TRCP-KLH antibodies and titration of antigen from mouse and rabbit sera. Before and after immunization protocols (see Figure 1), blood samples were taken to detect specific IgG anti-TCRP-KLH antibodies in the serum of animals immunized with the bioconjugate. An indirect ELISA was performed sensitizing plates from to μ g/mL of TCRP-KLH. (a) A pool of mice sera was diluted 1 : 1,000. (b) Serum from rabbit was diluted 1 : 10,000. Technical triplicates were carried out. .
(a) Mouse |
(b) Rabbit |