Journal of Immunology Research / 2019 / Article / Fig 7

Research Article

Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®)

Figure 7

Effect of the capture antibody concentration in a sandwich ELISA. Rabbit IgG anti-TCRP-KLH was purified from hyperimmune serum through protein-A column, then it was tested at different concentration for sensitizing the ELISA plates; TCRP-BSA was used as antigen with fivefold increments ranging 0.01-1.25 μg/mL; the pool of mice was diluted 1 : 100. Full system depiction is shown in Figure 2. Technical triplicates were carried out. , .

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