Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®)

Figure 8

Detection of Transferon® peptides through a competition ELISA. The system used is depicted in Figure 2. The capture antibody (rabbit purified IgG anti-TCRP-KLH) was used at 80 μg/mL; meanwhile, antigen concentration (TCRP-BSA) was 0.25 μg/mL. Different concentrations of Transferon® (5, 500, and 50,000 μg/mL) or diluent (dotted line) were preincubated with three dilutions of the secondary antibody (pool of mice sera). Technical triplicates were carried out. .