Journal of Immunology Research / 2019 / Article / Fig 4

Research Article

Expression of LAIR-1 (CD305) on Human Blood Monocytes as a Marker of Hepatic Cirrhosis Progression

Figure 4

Effect of differentiation and activation on LAIR-1 expression in a macrophage-like in vitro model. The expression of LAIR-1 was analyzed by Western blot in total protein extracts from the HL-60 cell line undifferentiated or differentiated into macrophages with PMA treatment and unstimulated (Con, control) or stimulated with LPS or C. albicans 1 : 5 cell : yeast ratio. A representative Western blot is shown (a). The bands were quantified by densitometry and normalized to β-actin. The normalized value was referred to the undifferentiated control (normalized to 1) (b), to its own untreated control in undifferentiated HLA-60 cells (c) and in differentiated HL-60 cells (d). LAIR-1 expression was analyzed by flow cytometry. Representative histogram of HL-60 cells unstained and stained with anti-LAIR-1 antibody in undifferentiated and differentiated cells is shown (e). The cell surface LAIR-1 protein expression was quantified by measuring the MFI (median fluorescence intensity) after cell staining, and the effect of differentiation process (f) or the activation with LPS or C. albicans was analyzed in undifferentiated (g) or differentiated HL-60 cells (h). Data represented in histograms are the mean and SEM. Student’s -test: , , and .

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