IFN-γ Reduces Epidermal Barrier Function by Affecting Fatty Acid Composition of Ceramide in a Mouse Atopic Dermatitis Model

Figure 1

Cytokine expression in FAg-induced dermatitis: upregulation of IFN-γ. FAg or vehicle solution was applied to the auricles of NC/Nga mice twice a week for five times. Six hours after the fifth application on day 14, total RNA was extracted from the whole auricles and the expression of cytokines was determined using quantitative RT-PCR. “IL-12/IL-23” means IL-12 and/or IL-23 because p40, a common subunit to IL-12 and IL-23, was amplified. Expression of each cytokine is presented as a value relative to GAPDH mRNA. Data are presented as in nine (FAg) or ten (vehicle) mice and are from one experiment representative of two independent experiments. Two-sided Student’s t-test was used for pair-wise comparisons. and .