Figure 3: The effects of LPS have not been caused by a presence of DCs in NK cell fractions. (a) Gating strategy for the sorting of NK cell subpopulations. (b) Sorted CD57+ and CD57 NK cells. (c) Sorted CD57HLA-DR NK cells were able to respond independently to the LPS stimulation of HLA-DR+ cell presence. CD57HLA-DR and CD57HLA-DR+ NK cells were isolated by cell sorting, then mixed in indicated proportions and incubated with IL-2 (500 U/ml) and S-LPS (5 μg/ml) overnight. Results of one of three experiments performed on the blood samples from different donors are shown. Data are of triplicate wells.