The Role of O-Antigen in LPS-Induced Activation of Human NK Cells

Figure 3

The effects of LPS have not been caused by a presence of DCs in NK cell fractions. (a) Gating strategy for the sorting of NK cell subpopulations. (b) Sorted CD57⁺ and CD57^– NK cells. (c) Sorted CD57^–HLA-DR^– NK cells were able to respond independently to the LPS stimulation of HLA-DR⁺ cell presence. CD57^–HLA-DR^– and CD57^–HLA-DR⁺ NK cells were isolated by cell sorting, then mixed in indicated proportions and incubated with IL-2 (500 U/ml) and S-LPS (5 μg/ml) overnight. Results of one of three experiments performed on the blood samples from different donors are shown. Data are of triplicate wells.

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