Low-Dose Irradiation Differentially Impacts Macrophage Phenotype in Dependence of Fibroblast-Like Synoviocytes and Radiation Dose

Table 1

Antibodies used for flow cytometry-based FLS and macrophage phenotyping.

Antibody	Dilution	Manufacturer	Application
CD11b-FITC	1 : 100	BD Bioscience, Franklin Lakes, NJ, USA	FLS phenotyping
CD106/VCAM-1-FITC	1 : 100	eBioscience, Frankfurt, Germany	FLS phenotyping
CD54/ICAM-1-PE	1 : 400	eBioscience	FLS phenotyping
IgG2bκ-FITC	1 : 100	BD Bioscience	FLS phenotyping
IgG2aκ-FITC	1 : 100	BD Bioscience	FLS phenotyping
IgG2aκ-PE	1 : 400	BD Bioscience	FLS phenotyping
CD11b-FITC	1 : 500	BD Bioscience	Macrophage characterization
F4/80-eFluor660	1 : 750	eBioscience	Macrophage characterization
MHCII-eFluor450	1 : 1000	eBioscience	Macrophage characterization
CD80-PE	1 : 500	BD Bioscience	Macrophage characterization
CD86-perCP-Vio700	1 : 50	Miltenyi Biotec, Bergisch Gladbach, Germany	Macrophage characterization
CD206-BV605	1 : 20	BioLegend, San Diego, CA, USA	Macrophage characterization