Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases
G-derivative, SHS particle preparation, and immunization schedule. (a) Structural representation of the G-derivative (ImAG) constituent of the SHS particles. (b) Schematic representation of the preparation protocol for the formulations used. (c) Each mouse was intramuscularly immunized with 100 μg of the pDNAs with or without 0.1515 mM SHS in 100 μL of PBS (50 μL/leg) on days 0, 14, and 28. Mice were bled intracardially and euthanized by cervical dislocation on the 7th day after the third immunization, following the guidelines established by the IACUC and NIH.