Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases
Effects of coadministration of SHS particles in antigen-specific cellular immune responses examined by ELISpot assays. The groups of female BALB/c mice (/group) were immunized intramuscularly, three times with a two-week interval. One week after the last immunization, cell-mediated immunity was assessed by IFN-γ ELISpot assays. (a) IFN-γ ELISpot assay of splenocytes stimulated with HIV-1 Gag (consensus subtype B) peptide mix (). (b) IFN-γ ELISpot assay of splenocytes stimulated with VVWR A27 peptide mix (pA27L study) (). (c) IFN-γ ELISpot assay of splenocytes stimulated with VVWR A27 peptide mix (pOD1A27Lopt study) (). Data are shown as the of at least three independent immunization studies with three replicates. Con A data is not shown as it goes out of scale.