Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases

Figure 5

IgG isotype antibody production due to administration of pDNAs and SHS particles as determined through ELISA. The groups of mice (/group) were DNA-based immunized i.m., three times with a two-week interval to evaluate antibody responses. Seven days after the last immunization, we prepared serum dilutions from each group of mice and led it to react with HIV-1 Gag p24 or VVWR A27 recombinant proteins. (a) IgG2a response against HIV-1 Gag p24 protein (). (b) IgG2a response against VVWR A27 protein (pA27L study) (). (c) IgG2a response against VVWR A27 protein (pOD1A27Lopt study) (). (d) IgG1 response against HIV-1 Gag p24 protein (). (e) IgG1 response against VVWR A27 protein (pA27L study) (). (f) IgG1 response against VVWR A27 protein (pOD1A27Lopt study) (). Data are shown as the of at least three independent immunization studies with three replicates.

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