Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells

Figure 4

Expression of mRNA for specific cell marker mRNA in macrophage depletion. RQ values are shown from macrophage-depletion experiment 2; RAG2 ^-/-γc^-/- mice injected with huPBMCs with macrophage depletion due to clodronate-containing liposomes (indicated as +) and RAG2 ^-/-γc^-/- mice injected with huPBMCs without this depletion (indicated as -), for different human cell types, including FoxP3 (Tregs), GATA3 (Th2 cells), Tbet (Th1 cells), RORC (Th17 cells), CD4 (T helper cells), CD8 (cytotoxic T-cells), and CD138 (plasma cells) as well as for murine CD68 (murine macrophages). Human CD68 showed cycle threshold values above 30 only and human macrophages were therefore considered as virtually not present.

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