Research Article
Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells
Table 1
Antibodies used for immunohistochemical detection of human and murine cells after huPBMC injection in RAG2-/-γc-/- mice.
| | Primary antibody | Company | Animal | Mono/poly | Clone | Lot | Dilution | Pretreatment |
| | Hu-CD2 | Novocastra, United Kingdom | Mouse | Monoclonal | AB75 | 127119 | 1 : 160 | EDTA | | Hu-CD4 | Monosan Xtra, Netherlands | Mouse | Monoclonal | 4B12 | 247919 | 1 : 200 | EDTA | | Hu-CD8 | DakoCytomation, Denmark | Mouse | Monoclonal | C8/144B | 51182 | 1 : 200 | Citrate | | Hu-CD20 | DakoCytomation, Denmark | Mouse | Monoclonal | L26 | 83 | 1 : 400 | Citrate | | Hu-CD79a | DakoCytomation, Denmark | Mouse | Monoclonal | JCB117 | 42791 | 1 : 200 | Citrate | | HuCD138 | Serotec, Germany | Mouse | Monoclonal | B-B4 | 605 | 1 : 1000 | Citrate | | Hu-kappa | DakoCytomation, Denmark | Rabbit | Polyclonal | . | 44399 | 1 : 10000 | Citrate | | Hu-lambda | DakoCytomation, Denmark | Rabbit | Polyclonal | . | 120 | 1 : 20000 | Citrate | | Hu-plasmacell | DakoCytomation, Denmark | Mouse | Monoclonal | VS38c | 38925 | 1 : 400 | EDTA | | HuCD68 | Novocastra, United Kingdom | Mouse | Monoclonal | KP1 | 211708 | 1 : 800 | Citrate | | Hu-FoxP3 | eBioscience, United Kingdom | Rat | Monoclonal | PCH101 | E021753 | 1 : 200 | Citrate | | Mouse-CD68 | Hycult Biotech, Netherlands | Rat | Monoclonal | FA-11 | 4817M21 | 1 : 50 | None |
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