Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells
Table 2
Abbreviations used in qPCR analysis.
(a)
Abbreviations used in qPCR analysis regarding specific cytokine/protein/gene function
TGF-β/TFGb
Transforming growth factor beta
Multifunctional cytokine known for its role in regulating inflammatory processes and involved in proliferation, activation, and chemotaxis of many immune cells
CTLA-4
Cytotoxic T-lymphocyte-associated antigen 4
Protein receptor involved in downregulation of immune responses, expressed on T cells
GAPDH
Glyceraldehyde-3-phosphate dehydrogenase
Protein coding gene, known as “housekeeping” gene
IFN-γ
Interferon gamma
Important regulating cytokine in inflammatory processes, with immunostimulatory and immunomodulating effects
CXCR4
Chemokine receptor type 4
Receptor for stromal derived factor 1 (chemotactic for lymphocytes and important for hematopoietic homing of stem cells)
FGF2
Fibroblast growth factor 2
Fibrosis-related factor with mitogenic effects on fibroblasts.
PDGF
Platelet derived growth factor
Fibrosis related factor with mitogenic effects on fibroblasts
EGF
Epidermal growth factor
Promotor of TGF-β-related fibrosis
BMP4
Bone morphogenetic protein 4
Proinflammatory gene with profibrotic potential
CTGF
Connective tissue growth factor
Profibrotic cytokine
(b)
Abbreviations used in qPCR analysis regarding specific cell types
FoxP3
Abbreviation for Forkhead protein 3, used as signature for regulatory T cells (Tregs)
GATA3
Transcription factor involved in T helper 2 (Th2) cell differentiation
Tbet
Transcription factor associated with T helper 1 (Th1) cell differentiation
