Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep

Table 1

Sequences of the primers used for relative gene quantification (RT-qPCR) in Morada Nova lambs resistant or susceptible to Haemonchus contortus infection, including: accession number, amplicon size, exon boundary covered, efficiency (), determination coefficient (), and slope.

Gene	Access number	Sequence (5-3)	Amplicon size (bp)	Exon boundary	(%)		Slope
GAPDH	NM_001190390.1	F: CAAGCTCATTTCCTGGTACGAC; R: TCTCTCTTCCTCTCGTGCTCCT	131	10/11	99.136	0.999	-3.343
YWHAZ	NM_001267887.1	F: CTGAGAAAGCCTGCTCTCTTGC; R: GGTATCCGATGTCCACAATGTC	143	5/6	102.340	0.999	-3.267
TLR2	NM_001048231.1	F: CTCCCACTTCCGTCTCTTTGAT; R: CTCCAGGTAGGTCCTGGTGTTC	133	Intronless	96.378	0.999	-3.412
TLR4	NM_001135930	F: ACCCTTGCGTACAGGTTGTTC; R: ATGGCTGCCTAAATGTCTCAGG	137	1/2	100.710	0.964	-3.300
TLR7	NM_001135059.1	F: TTGAGAAGCCCCTTCAGAAGTC; R: TCAGACACTGCCAGAAGTACGG	117	None	101.823	0.994	-3.279
TLR10	NM_001135925.1	F: GTGGTTATCATGCTCGTTCTGG; R: TCTTCCTAACCCTGAGCCATGT	118	Intronless	95.298	0.992	-3.440
NFKBIA	NM_001166184.1	F: CGAGACTTTCGAGGAAATACCC; R:GACACGTGTGGCCATTGTAGTT	141	3/4	95.848	0.999	-3.420
IKBKB	XM_015104530.1	F: AGGCTGCCGAGAAGAGTGAC; R: CAAACTCTGGTCCTGCTCCTTC	104	23/24	92.421	0.986	-3.518
iNOS	AF223942.1	F: CACCTCTACTGGGAGGAGATGC; R: GAACATAGACCTTGGGCTGGTC	102	24/25	99.905	0.999	-3.324
IL1β	NM_001009465.2	F: AGTGGTGTTCTGCATGAGCTTC; R: CAGGGTCGGTGTATCACCTTTT	124	4/5	100.440	0.997	-3.311
TNFα	NM_001024860	F: CTCAGGTCATCTTCTCAAGCCT; R: GAGGGCATTGGCATACGAG	108	2/3	94.086	0.983	-3.472
IL10	NM_001009327.1	F: CTTTAAGGGTTACCTGGGTTGC; R: TCACGTGCTCCTTGATGTCAG	110	2/3	102.305	0.995	-3.268
TGFβ	NM_001009400	F: CAGCTCCACAGAAAAGAACTGC; R: GTGTCCAGGCTCCAGATGTAGG	144	5/6	98.993	0.994	-3.346
C7	XM_012096998.2	F: CTATGAATGTGGGTCCTCCTTG; R: CTCCCTACCAGCCACAGTGTAA	130	16/17	102.74	0.990	-3.258
CFI	XM_004009622.3	F: ATGGAGTGTGCAGGTACAGATG; R: CTCACAATACCCCAAACGTAAG	113	14/15	99.818	0.997	-3.326