Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype
(a) Expansion kinetics of PBMCs activated with CD3/CD28 antibodies. Cells were cultured in media supplemented with 5% FBS, ABS, or HPL in the presence of 100 IU/mL IL2. (b) Total fold expansion at day 14 of culture. of three donors is shown. . (c) Mean viability of cells expanded in culture media supplemented with FBS, ABS, or HPL. (d) Percentage of lymphocyte subsets after 14 days of PBMCs cultured with different media supplements.
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