Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype
(a) Representative flow cytometry analysis of expanded T cells. The expression of CCR7 and CD45RO markers was assessed on day 14 of culture for CD4+- or CD8+-gated T cells. (b, c) Percentage of CCR7+/CD45RO- (TN) and CCR7+/CD45RO+ (TCM) fractions in CD4+ and CD8+ T cells, respectively. of three donors is shown. . (d, e) Representative flow cytometry analysis and percentage of CD62L-positive T cells at day 14 of culture. of three donors is shown. .
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