Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro

Figure 2

Reduction of inflammatory response induced LPS in HepG2 cells. Both normal and free fatty acid-treated HepG2 cells were stimulated with total cellular fluid (50 μl/ml) of LABs for 48 h, then poststimulated with LPS for 3 h and 12 h. The expression of inflammatory cytokines (IL-6, CXCL8, CCL2, and TNF-α) was determined by real-time polymerase chain reaction (RT-PCR). HepG2 cell treated with LPS was used as control. Different superscript letters indicate significant differences at the 0.05 level.