Research Article
Analysis of Toll-Like Receptors in Human Milk: Detection of Membrane-Bound and Soluble Forms
Table 1
List of antibodies used in this study.
| | Antibody | Source | Producer | Dilution |
| | Anti-TLR1 | Polyclonal rabbit antibody, directed against a recombinant peptide from human TLR1 (STJ25862) | St John’s Laboratory | 1 : 500 | | Anti-TLR2 | Polyclonal rabbit antibody (H-175), directed against aa 180-354 of human TLR2 (sc-10739) | Santa Cruz Biotechnology, Inc. | 1 : 500 | | Anti-TLR4 | Polyclonal rabbit antibody (H-80), directed against aa 242-321 of human TLR4 (sc-10741) | Santa Cruz Biotechnology, Inc. | 1 : 500 | | Anti-TLR6 | Polyclonal rabbit antibody directed against a peptide of 13 aa near the central part of human TLR6 (PRS3653) | Sigma-Aldrich | 1 : 1000 | | Anti-β-actin | Polyclonal rabbit antibody (20536-1-AP) | Proteintech | 1 : 2000 | | Anti-rabbit-AP | Polyclonal goat anti-rabbit antibody conjugated with alkaline phosphatase (A3687) | Sigma-Aldrich | 1 : 3000 |
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